Abstract
Hyperglycemia is treated with canagliflozin (CANA), for determination of CANA in human plasma, simple, accurate, precise, and reproducible RP-HPLC with mass spectrometry method was developed and validated via protein precipitation method. The average rate of CANA revival was 89.79 percent. There were no interfering endogenous components detected during the analyte’s retention time. Bioavailability and bioequivalence investigations necessitating determination of CANA in human plasma can now make use of a straightforward and reproducible LC-MS-MS technique.
Keywords: Bioanalytical, LC-MS/MS, Canagliflozin, Protein precipitation, Validation.